Table 4.

Mass concentration values of human genomic DNA samples obtained using the different methods. Absorbance values and derived mass values of the human genomic DNA samples from a double-beam (DB) spectrophotometer, microliter volume (MV) spectrophotometers, microvolume (MV) plate reader, fluorescent dye binding, and digital PCR assays.

Test method	Absorbance	Mass (ng/μl)
Absorbance DB Spec.	1.029 (.051, N = 9)	51.5 (2.6)*
Absorbance MV Spec. A	1.04 (0.05, N = 29)	52.0 (2.5)*
Absorbance MV Spec. A (0.2 M NaOH)	0.71 (0.01, N = 6)	52.4 (0.4)**
Absorbance MV Spec. B	1.012 (0.086, N = 30)	50.6 (4.3)*
Absorbance MV Spec. C	0.973 (0.050, N = 6)	48.7 (2.5)*
Absrobance MV Plate	1.011 (0.036, N = 26)	50.6 (1.8)*
Compact Flurorimeter (Broad Range Lambda DNA manufacturer’s standard)		38.0 (0.3, N = 6)
Compact Flurorimeter (High Sensitivity Lambda DNA manufacturer’s standard)		46.5 (0.5, N = 4)
Compact Flurorimeter (High Sensitivity Calf Thymus DNA standard)		46.9 (0.7, N = 4)
Compact Flurorimeter (AccuGreen, Calf Thymus DNA manufacturer’s standard)		52.5 (0.7, N = 36)
Microplate Fluorimeter (AccuBlue Dye A, Calf Thymus manufacturer’s standard)		54.6 (1.8, N = 3)
Microplate Fluorimeter (AccuBlue Dye B, Calf Thymus manufacturer’s standard)		49.9 (1.0, N = 22)
Microplate Fluorimeter (AccuClear Dye, Calf Thymus manufacturer’s standard)		47.6 (0.8, N = 18)
Droplet Digital PCR Assays***		53.9 (2.5, N = 81)

^*The mass concentrations were calculated using the assumption that 1 Absorbance Unit at 260 nm and 1 cm pathlength equals 50 ng/μl. The values in the parentheses are 1 standard deviation.

^**The DNA sample was denatured by adding an equal volume of 0.4 mol/L NaOH and converted to mass by multiplying a dilution factor of 2 with the assumption that 1 Absorbance Unit at 260 nm and 1 cm pathlength equals 37 ng/μl.

^***Calculated using the assumptions shown in Table 5.