Table 5.
Mass concentration of the human genomic DNA sample calculated from droplet digital PCR data with different assumptions of the droplet size.
| Size (Base pairs) | Genome mass (pg) | Droplet size (nl) | Copies/μl (SD) | Mass concentration (ng/μl) |
|---|---|---|---|---|
| 3.01 × 109 | 3.3 | 0.7349* (uncertainty 2.3%, k = 2) | 16,323 (759, N = 81) | 53.9 (2.5) |
| 3.01 × 109 | 3.3 | 0.7681* (uncertainty 2.3%, k = 2) | 15,610 (715, N = 81) | 51.5 (2.4) |
| 3.01 × 109 | 3.3 | 0.85 | 14,107 (646, N = 81) | 46.6 (2.1) |
The values in parentheses are 1 standard deviation of the data from the MET, DCK, and EIF5B gene assays (data shown in Figure 4). The average haploid genome mass was calculated using an average molecular weight of the base pairs of 660, as detailed in the text.