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N-terminal peptide mapping and ion fragmentation. (A) Sketch of the N-terminal peptide fragment following trypsin digestion of NAcαS, with overlaid b- and y-ion maps. (B) Tables with experimental and theoretical data for both the b-ion and y-ion series of tetrameric vs monomeric NAcαS. (C) Annotated CID mass spectrum for tetrameric NAcαS demonstrating sequence match; y3 and y4 are denoted to highlight the high mass accuracy (<1 ppm).
