Figure 1.

Study outline, body weight development, and body composition of Göttingen minipigs fed a HFHE-diet for 70 weeks. (A) Study outline: age of minipigs from the lean (L, n = 5) and obese group (O, n = 21) at the study start: 13 months, FBS: fasting blood sampling, IVGTT: intravenous glucose tolerance test, HR: heart rate, TT: treatment trial, DXA: dual-energy X-ray absorptiometry, MRT: magnetic resonance tomography. (B) Body weight gain of minipigs from the lean and obese group. (C–F) Body composition evaluated by DXA prior to HFHE-diet feeding (0) and at feeding week 18, 30 and 54, (C) Relative fat mass (%), (D) Absolute lean mass (kg), (E) Absolute bone mineral content (BMC, kg), (F) Bone mineral density (g/cm²). (G, H) Representative MRT images of a lean (G) and an obese (H) minipig at the level of the kidneys showing subcutaneous and visceral obesity. Data are means ± SEM; *: p<0.05, **: p<0.01, ***: p<0.001. Significant differences within the groups are indicated by different letters, differences between lean and obese animals at the respective time point are indicated by asterisks.