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. 2018 Jun 28;16:180–190. doi: 10.1016/j.molmet.2018.06.015

Figure 4.

Figure 4

Histopathological findings in 3-year-old, ovariectomized Göttingen minipigs fed a HFHE-diet for 70 weeks. HE-stained paraffin-sections (A–K); Oil-red stained cryo-sections (L and inset to I). A–D: Histomorphology of unaltered subcutaneous adipose tissue of the back (scB A, B) and of retroperitoneal adipose tissue of the lateral abdominal wall (rpAW, C, D) of lean, need-based HFHE-fed control pigs and obese HFHE-fed pigs without evident inflammatory or necrotic alterations. Note the drastically increased section profile areas of retroperitoneal adipocytes in obese pigs (D). Bars = 100 μm. E, F: Necrotic and inflammatory alterations in the retroperitoneal adipose tissue of obese HFHED-fed pigs. Bars = 100 μm. E: Moderate interstitial, perivascular lymphocytic infiltration, edema, hypertrophy of vascular walls and capillary endothelia, focal hemorrhage, and mild interstitial fibrosis. F: Extensive fat cell necrosis with saponification, cholesterol-crystal formation, and severe granulomatous inflammation (macrophages and numerous multinucleate giant cells). G–L: Visceral organ lesions in obese HFHED-fed pigs. G, H: Pancreas. G: Severe peri-pancreatic necrosis of fat (right side). Bar = 1 mm. H: Detail enlargement, illustrating the loss of acinar exocrine pancreatic parenchyma with (regenerative) proliferation of pancreatic duct epithelia adjacent to an area of fat tissue necrosis (right side). Asterisk marks the section profile of an endocrine pancreatic islet. Bar = 100 μm. I: Hepatic foam cell granuloma of lipid-loaded macrophages. Inset to I: Positive fat staining (oil-red, fat stains orange-red) in a cryo-section of liver tissue. Bars = 100 μm. J: Myocardial interstitial lipomatosis. Asterisk: Myocardial Purkinje-cells. Bars = 100 μm. K–L: Pulmonary lymphangiectasia and lipidosis. Bar = 100 μm. L: Positive fat-staining (oil-red, fat stains orange-red) in a cryo-section of lung tissue. Inset to L: Immunohistochemical detection (brown color) of LYVE1 (lymphatic marker). Asterisk: Blood capillary. Paraffin section. Chromogene: DAB. Nuclear counterstain: Hemalaun. Bar = 25 μm.