KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal V5-HRP (clone V5–10) | Sigma | Cat#: V2260; RRID: AB_261857 |
| Rabbit polyclonal anti-PB2 | Mehle and Doudna, 2008 | N/A |
| Goat polyclonal anti-RNP | BEI Resources | Cat#: NR-3133 |
| Mouse monoclonal anti-FLAG (clone M2) | Sigma | Cat#: F1804; RRID: AB_262044 |
| Rabbit polyclonal anti-V5 | Bethyl | Cat#: A190–120A; RRID: AB_67586 |
| Goat anti-mouse Alexa Fluor 594 | Invitrogen | Cat#: A-11032; RRID: AB_2534091 |
| Goat anti-rabbit Alexa Fluor 488 | Invitrogen | Cat#: A-11008; RRID: AB_143165 |
| Agarose resin: anti-FLAG (clone M2) | Sigma | Cat#: A2220 |
| Bacterial and Virus Strains | ||
| E. coli: strain BL21 Rosetta 2(DE3)pLysS | Novagen | Cat#: 70956 |
| Influenza A virus: A/WSN/33 (H1N1; WSN) | Neumann et al., 2005 | N/A |
| Influenza A virus: A/green-winged teal/Ohio/175/1986 (H2N1; S009) |
Mehle and Doudna, 2009 | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Magnetic beads: MagneGST glutathione particles | Promega | Cat#: V8611 |
| MMLV Reverse Transcriptase | Kirui et al., 2016b | N/A |
| T4 Polynucleotide Kinase | NEB | Cat#: M0201L |
| ECL | GE Healthcare | Cat#: GERPN2236 |
| Critical Commercial Assays | ||
| MycoAlert | Lonza | Cat#: LT07–218 |
| Renilla luciferase assay system | Promega | Cat#: E2810 |
| Experimental Models: Cell Lines | ||
| Human: 293T | ATCC | CRL-3216 |
| Human: A549 | ATCC | CCL-185 |
| Cow: MDBK | ATCC | CCL-22 |
| Dog: MDCK | ATCC | CCL-34 |
| Chicken: LMH | ATCC | CRL-2117 |
| Experimental Models: Organisms/Strains | ||
| WSN-PB2-FLAG | Kirui et al., 2016b | N/A |
| WSN-PB2-K627E-FLAG | This paper | N/A |
| S009 SRK | Mehle and Doudna, 2009 | N/A |
| Oligonucleotides | ||
| Primer: NP77F-TGATTTCGATGTCACTCTGTGAGT | Kirui et al., 2016b | N/A |
| Primer: NP77R-GCAGGGTAGATAATCACTGACAGAG | Kirui et al., 2016b | N/A |
| Primer: 5 s- ACCCTGCTTAGCTTCCGAGA | Kirui et al., 2016b | N/A |
| Recombinant DNA | ||
| Plasmid: pENTR chANP32A29 | This paper | N/A |
| Plasmid: pENTR chANP32AD | This paper | N/A |
| Plasmid: pENTR chANP32A33 | This paper | N/A |
| Plasmid: pDONR221 huANP32A | DNASU | HsCD00042415 |
| Plasmid: pDONR221 huANP32A+29 | This paper | N/A |
| Plasmid: pENTR PB2 627 domain | This paper | N/A |
| Plasmid: pENTR PB2 627-NLS | This paper | N/A |
| Plasmid: pcDNA6.2/V5-DEST | Invitrogen | Cat#: 12489027 |
| Plasmid: pENTR/TEV/D-TOPO | Invitrogen | Cat#: K253520 |
| Plasmid: pHGGWA | Busso et al., 2005 | N/A |
| Plasmid: pHMGWA | Busso et al., 2005 | N/A |
| Plasmid: pLX304 | Yang et al., 2011 | Addgene Cat#: 25890 |
| Plasmid: pHH21 vNA-Luc | Mehle and Doudna, 2008 | N/A |
| Plasmid: pHH21 3–8 vNA-Luc | This paper | N/A |
| Plasmid: pHH21 NP-77 | Kirui et al., 2016b | N/A |
| Plasmid: pcDNA PB1a | This paper | N/A |
| Software and Algorithms | ||
| HISAT2 v2.1.0 | Kim et al., 2015 | www.ccb.jhu.edu/people/infphilo |
| BBDuk v37.75 | Bushnell, 2015 | https://sourceforge.net/projects/bbmap/ |
| FastQC v0.11.5 | Andrews, 2010 | https://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ |
| Samtools v1.6 | Li et al., 2009 | http://www.htslib.org/download/ |
| IGV v2.4.4 | Katz et al., 2015 | https://software.broadinstitute.org/software/igv/ |