Figure 6. Representative images of different behaviors displayed during 15 min test period.

A. Immobility; B. Burying–the rat is pushing bedding toward the probe with the forepaws. C. Burrowing into the bedding (scored as burying). This is often accompanied by flicking or pushing bedding toward the probe. D. Exploratory behavior (not scored); E. Immobility near probe (not scored); F. Immobility while attending to the probe (not scored). Dashed line indicates the midline of the testing area, which serves as the cutoff for where immobility may be scored (i.e., in testing area away from the probe).