Skeletal muscle mitochondrial respiration during SUIT1 (A), SUIT2 (B), and ADP titration protocol (C). Vmax (D) and apparent Km of ADP (E) were calculated from the ADP titration curve. Comparison of OXPHOS (F) and ETS (G) between SUIT1, SUIT2, and ADP titration (ADP) protocols before (Pre) and after (Post) CON and CTR. An average of the P/E ratio for each protocol (SUIT1, SUIT2, and ADP) (H) are presented with no differences between study days. Data are expressed as means ± SE of technical duplicates. Because of a limited sample, the Post-CON for the ADP titration was not run in duplicate.