Skip to main content
. Author manuscript; available in PMC: 2018 Sep 26.
Published in final edited form as: Mol Cancer Ther. 2017 Jun 15;16(9):2022–2034. doi: 10.1158/1535-7163.MCT-17-0098

Table 2. Sequencing of BRCA1 in SUM149.B1.S*.

Cell Line Reference sequence for BRCA1
CAATCCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACACTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAAG		 CRISPR-Induced mutation Subcloned colonies sequenced (#) Estimated allele frequency
SUM149 CAA -CCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAA G N/A 20 1.00
SUM149.B1.3* CAA-CCTAG———————————TAAG 80-bp Deletion 20 0.66
CAA-CCTAGCCTTCCAAGAGAAGAAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGACCCCAAAGATCTCATGTTAA G SUM149 {2288delT] 0.33