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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Protein Eng Des Sel. 2018 May 1;31(5):173–179. doi: 10.1093/protein/gzy015

Figure 1. Cloning, expression and characterization of IL2-αCD38-αCD38-scTRAIL.

Figure 1

(a) Schematic representation of the cloning strategy and of the domain assembly. (b) Size exclusion chromatography profile of IL2-αCD38-αCD38-scTRAIL (120 kDa, black line), IgG (150 kDa, blue line) and SIP (80 kDa, green line) were used as controls for the column’s calibration. (c) SDS-PAGE analysis: MW, molecular weight; NR, nonreducing conditions; R, reducing conditions. (d) ESI-MS profile. (e) Amino acid sequence of IL2-αCD38-αCD38-scTRAIL. Starting from the N-terminus: human IL2, the αCD38 antibody in tandem diabody format and TRAIL in single chain format.