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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Protein Eng Des Sel. 2018 May 1;31(5):173–179. doi: 10.1093/protein/gzy015

Figure 3. Activity against MM cells.

Figure 3

(a) Flow cytometric evaluation of the binding of IL2-αCD38-αCD38-scTRAIL to RPMI8226 cells, detected with an anti-IL2 reagent. (b) Selective killing of RPMI8226 cells 24 hours after incubation with 25 nM IL2-αCD38-αCD38-scTRAIL. Dual-color flow cytometry analysis for CD138-APC and 7-AAD indicates that the fusion protein induced cell death (revealed by 7-AAD staining) in CD138-positive cells. Quadrants were set in order to differentiate CD138+ cells from unstained cells. (c) Selective killing of freshly isolated MM patient cells, upon 16 h incubation with the fusion protein.