Figure 2.

Nanopore single-molecule discrimination of the BRAF V600E mutant allele/probe duplex containing a nanolock. (a,b) Sequences of the wild-type target/probe (T^WT•P, a) and the V600E mutant/probe (T^V600E•P, b) duplexes. The T^V600E•P duplex can form an interstrand nanolock on the T-T mismatch in the presence of Hg²⁺ (b); (c,d) Similar current block patterns and model cartoons for one-step unzipping of T^WT•P (c) and T^V600E•P (d) in the absence of Hg²⁺; (e,f) Distinct current block patterns and model cartoons for one-step unzipping of T^WT•P (e) and stepwise unzipping of T^V600E•P carrying a nanolock (f) in the presence of Hg²⁺.