Figure 3.

Detection of the BRAF V600E mutant target T^V600E in the mixture with the T^WT•P duplex. (a) Current traces showing the e-marker signals for the T^V600E•P with a nanolock (solid triangles) and nonmarker blocks for the T^WT•P duplex and free T^V600E•P duplex without nanolock (empty triangles); (b) Increase of the frequency ratio of e-marker versus nonmarker blocks (N+/N−) as a function of the T^V600E percentage in the mixture.