Table 1. Primers and relative information of the reference and target genes.
Gene symbol | Primer sequence (5'-3') | Amplicon length (bp) | Tm(°C) | Source |
---|---|---|---|---|
YWHAZ | F: ATGCAACCAACACATCCTATC | 178 | 62 | Erkens et al. (2006) |
R: GCATTATTAGCGTGCTGTCTT | ||||
UBC | F: TTGAGGGGAGGTTTCTAA | 82 | 58.4 | M18159 |
R: GAATGCAACAACTTTATTGA | ||||
TBP | F: GATGGACGTTCGGTTTAGG | 124 | 55.9 | Erkens et al. (2006) |
R: AGCAGCACAGTACGAGCAA | ||||
RPL32 | F: AGCCCAAGATCGTCAAAAAG | 165 | 55 | NM_001001636 |
R: TGTTGCTCCCATAACCAATG | ||||
RPL19 | F: GCTTGCCTCCAGTGTCC | 82 | 62 | AF435591 |
R: GTTGGCGTTGGCGATTT | ||||
PPIA | F: GGGAGAAAGGATTTGGTTAT | 175 | 62 | NM_214353 |
R: ATGGACAAGATGCCAGGAC | ||||
PPARGC1A | F: CCTGCATGAGTGTGTGCTCT | 107 | 59 | Muráni et al. (2007) |
R: CTCAGAGTCCTGGTTGCACA | ||||
PGK1 | F: GGGCTAAGCAGATTGTATG | 180 | 52.8 | NM_001099932 |
R: TGACTTTATCCTCCGTGTT | ||||
HSPCB | F: GGCAGAAGACAAGGAGAAC | 131 | 55.9 | AF288819 |
R: CAGACTGGGAGGTATGGTAG | ||||
CANX | F: CAATGATGGATGGGGTCTGAA | 135 | 60 | Muráni et al. (2007) |
R: AACACAGGTAATGCCACAGTCAA | ||||
ALDOA | F: GAACCAACGGCGAGACAA | 142 | 55.6 | AY359812 |
R: ATGATGGCGAGGGAGGAG | ||||
5S | F: GCCCGATCTCGTCTGATCT | 93 | 54 | AF329851 |
R: AGCCTACAGCACCCGGTATT | ||||
18S | F: CCCACGGAATCGAGAAAGAG | 125 | 53 | Wang et al.(2016) |
R: TTGACGGAAGGGCACCA | ||||
B2M | F: CAAGATAGTTAAGTGGGATCG | 161 | 60 | Wang et al.(2016) |
R: TGGTAACATCAATACGATTTC | ||||
B-actin | F: GGATGCAGAAGGAGATCACG | 134 | 51 | Wang et al.(2016) |
R: ATCTGCTGGAAGGTGGACAG | ||||
GAPDH | F: CCTTCCGTGTCCCTACTGC | 195 | 55.9 | AF017079 |
R: CATCAAAGGTAGAAGAGTGAGTGTC | ||||
HMBS | F: AGGATGGGCAACTCTACCTG | 83 | 59.5 | Wang et al.(2016) |
R: GATGGTGGCCTGCATAGTCT | ||||
HPRT | F: GGACTTGAATCATGTTTGTG | 91 | 59.5 | Wang et al.(2016) |
R: CAGATGTTTCCAAACTCAAC | ||||
ALP | F: CTAAAGGGGCAGATGAATGG | 105 | 55.9 | Lackeyram et al.(2010) |
R: CACCTGTCTGTCCACGTTGT |