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. 2018 Sep 26;13(9):e0204583. doi: 10.1371/journal.pone.0204583

Table 1. Primers and relative information of the reference and target genes.

Gene symbol Primer sequence (5'-3') Amplicon length (bp) Tm(°C) Source
YWHAZ F: ATGCAACCAACACATCCTATC 178 62 Erkens et al. (2006)
R: GCATTATTAGCGTGCTGTCTT
UBC F: TTGAGGGGAGGTTTCTAA 82 58.4 M18159
R: GAATGCAACAACTTTATTGA
TBP F: GATGGACGTTCGGTTTAGG 124 55.9 Erkens et al. (2006)
R: AGCAGCACAGTACGAGCAA
RPL32 F: AGCCCAAGATCGTCAAAAAG 165 55 NM_001001636
R: TGTTGCTCCCATAACCAATG
RPL19 F: GCTTGCCTCCAGTGTCC 82 62 AF435591
R: GTTGGCGTTGGCGATTT
PPIA F: GGGAGAAAGGATTTGGTTAT 175 62 NM_214353
R: ATGGACAAGATGCCAGGAC
PPARGC1A F: CCTGCATGAGTGTGTGCTCT 107 59 Muráni et al. (2007)
R: CTCAGAGTCCTGGTTGCACA
PGK1 F: GGGCTAAGCAGATTGTATG 180 52.8 NM_001099932
R: TGACTTTATCCTCCGTGTT
HSPCB F: GGCAGAAGACAAGGAGAAC 131 55.9 AF288819
R: CAGACTGGGAGGTATGGTAG
CANX F: CAATGATGGATGGGGTCTGAA 135 60 Muráni et al. (2007)
R: AACACAGGTAATGCCACAGTCAA
ALDOA F: GAACCAACGGCGAGACAA 142 55.6 AY359812
R: ATGATGGCGAGGGAGGAG
5S F: GCCCGATCTCGTCTGATCT 93 54 AF329851
R: AGCCTACAGCACCCGGTATT
18S F: CCCACGGAATCGAGAAAGAG 125 53 Wang et al.(2016)
R: TTGACGGAAGGGCACCA
B2M F: CAAGATAGTTAAGTGGGATCG 161 60 Wang et al.(2016)
R: TGGTAACATCAATACGATTTC
B-actin F: GGATGCAGAAGGAGATCACG 134 51 Wang et al.(2016)
R: ATCTGCTGGAAGGTGGACAG
GAPDH F: CCTTCCGTGTCCCTACTGC 195 55.9 AF017079
R: CATCAAAGGTAGAAGAGTGAGTGTC
HMBS F: AGGATGGGCAACTCTACCTG 83 59.5 Wang et al.(2016)
R: GATGGTGGCCTGCATAGTCT
HPRT F: GGACTTGAATCATGTTTGTG 91 59.5 Wang et al.(2016)
R: CAGATGTTTCCAAACTCAAC
ALP F: CTAAAGGGGCAGATGAATGG 105 55.9 Lackeyram et al.(2010)
R: CACCTGTCTGTCCACGTTGT