. 2018 Sep 26;13(9):e0204583. doi: 10.1371/journal.pone.0204583

Table 3. A comparison of CT values of the 18 reference genes at different GITs and growth stages.

Gene	GIT				Age (Post-weaning)				SEM			P-value
Gene	Duodenum	Jejunum	Ileum	Colon	Day 0	Day 7	Day 14	Day 21	GIT	Age	GIT*Age	GIT	Age	GIT*Age
YWHA	23.61	23.62	23.03	23.61	24.09^a	23.44^b	23.47^b	22.89^c	0.185	0.185	0.369	0.065	<0.001	<0.001
UBC	20.07^b	20.01^b	19.69^b	20.61^a	19.62^c	20.26^ab	20.63^a	19.86^bc	0.189	0.189	0.379	0.010	0.002	<0.001
TBP	28.09^a	27.07^b	27.01^b	27.49^ab	28.57^a	26.84^bc	27.60^b	26.65^c	0.292	0.292	0.585	0.041	<0.001	0.003
RPL32	18.73	18.46	18.87	18.05	18.11^b	18.33^b	19.29^a	18.38^b	0.302	0.302	0.605	0.237	0.037	0.062
RPL19	21.39^a	20.41^b	19.68^c	20.91^ab	20.61^ab	20.69^ab	20.93^a	20.17^b	0.185	0.185	0.371	<0.001	0.039	<0.001
PPIA	23.27	22.76	22.75	22.29	23.73^a	23.29^a	21.84^b	22.20^b	0.332	0.332	0.664	0.232	<0.001	<0.001
PPARGGIA	29.15	28.59	28.77	29.06	29.18^a	28.15^b	29.32^a	28.92^a	0.207	0.207	0.415	0.209	0.001	<0.001
PGK11	24.57^a	23.48^b	22.53^c	23.69^b	23.19^b	23.63^ab	24.21^a	23.23^b	0.217	0.217	0.435	<0.001	0.004	0.001
HSPCB	25.16^a	23.06^b	23.29^b	23.58^b	24.12^a	23.63^ab	24.11^a	23.23^b	0.28	0.28	0.561	<0.001	0.081	<0.001
CANx	25.93	25.46	25.3	25.57	25.90a	25.15b	26.28a	24.93b	0.259	0.259	0.518	0.361	0.001	<0.001
ALDOA	26.87^a	24.78^b	25.09^b	25.08^b	26.84^a	24.75^c	25.88^b	24.34^c	0.29	0.29	0.579	<0.001	<0.001	<0.001
5S	22.09^b	21.12^c	22.19^b	23.52^a	22.41^ab	21.68^b	22.01^b	22.83^a	0.252	0.252	0.503	<0.001	0.012	<0.001
18S	15.72^a	16.09^a	15.74^a	15.07^b	15.76^ab	16.03^a	15.49^ab	15.35^b	0.223	0.223	0.446	0.015	0.141	<0.001
B2M	15.78	15.67	15.53	15.74	15.92	15.72	15.47	15.61	0.092	0.064	0.103	0.333	0.102	0.081
B-^actin	17.99^ab	18.47^a	17.40^b	17.77^b	18.07	17.92	17.71	17.93	0.199	0.199	0.398	0.003	0.654	0.042
G^aPDH	24.96^b	27.23^a	25.22^b	23.82^b	27.33^a	24.49^b	22.95^b	26.46^a	0.648	0.648	1.296	0.004	<0.001	<0.001
HMBS	26.65	26.80	26.61	26.74	26.91	26.64	26.58	26.67	0.099	0.099	0.197	0.508	0.094	0.052
HPRT1	25.61^a	24.73^b	24.53^b	24.83^b	25.22^a	24.98^ab	24.71^b	24.79^b	0.11	0.11	0.22	<0.001	0.007	0.001

^a,b,c Mean values within a row with unlike superscript letters were significantly different (P< 0·05).