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. 2018 Sep 26;13(9):e0204119. doi: 10.1371/journal.pone.0204119

Fig 1. Characterization of liver injury caused by chronic-binge ethanol exposure.

Fig 1

A: Schematic presentation of the chronic-binge ethanol exposure protocol. C57BL/J mice were fed control (pair-fed) or ethanol diets for 10 days, followed by single gavage of maltose dextrose or ethanol on day 11, respectively. Animals were euthanized 9 hours after gavage. B: The composition of the experimental liquid diets. The SF diet was enriched in beef tallow fat and MCTs (18:82 ratio). The USF diet was enriched with corn oil. Soybean oil was used in both diets to provide essential free fatty acids. The control (SF and USF) diets contained 43% of calories from carbohydrate, 17% from protein, and 40% from fat. The SF and ethanol and USF and ethanol diets contained 35% of calories from ethanol to replace the calories from carbohydrate. C: The percent of saturated, monounsaturated and polyunsaturated fatty acids was 84.1%, 9.9%, and 6.6% in SF diet and, 13.1%, 25.0%, and 61.9% in USF diet, respectively. D: Fatty acid composition of experimental diets. E: Representative images of hepatic hematoxylin and eosin (H&E), Oil Red O and chloroacetate esterase (CAE) staining. Original magnification, x400, scale bars are 50 μm. Arrows indicate CAE-positive neutrophils.