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. 2018 Sep 19;11:5933–5941. doi: 10.2147/OTT.S164007

Figure 3.

Figure 3

OPN levels correlate with LDHA expression levels and lactate production in NSCLC cell lines.

Notes: (A) Data from the Gene Expression Omnibus repository DataSet Record GDS1650 indicated that the mRNA expression levels of OPN correlate with LDHA mRNA expression levels. (B) Western blot analysis showing OPN and LDHA protein levels following OPN knockdown using siRNAs. (C) Lactate production of A549 and SK-MES-1 cells was measured following transfection with siOPN-1/siOPN-2 or siNC; oxamate (lactate dehydrogenase inhibitor) treatment was used as control. (D) CDDP resistance of A549 and SK-MES-1 cells when treated with siRNAs, CDDP (5 µg/mL, 24 hours), or lactate (5 mM, 24 hours). **P<0.001, ***P<0.0001.

Abbreviations: CDDP, cisplatin; LDHA, lactate dehydrogenase A; NSCLC, non-small-cell lung cancer; OPN, osteopontin; oxamate, sodium oxamate; siNC, small interfering RNA negative control; siOPN, small interfering RNA targeting OPN; siRNA, small interfering RNA.