Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) |
BL21 (DE3) Star | QB3 Macrolab | ||
| Recombinant DNA reagent |
ttRNH* (plasmid) | 10.1021/bi982684h | n/a | |
| Recombinant DNA reagent |
Anc1* (plasmid) | 10.1371/journal.pbio.1001994 | n/a | |
| Recombinant DNA reagent |
Anc2* (plasmid) | 10.1371/journal.pbio.1001994 | n/a | |
| Recombinant DNA reagent |
Anc3* (plasmid) | 10.1371/journal.pbio.1001994 | n/a | |
| Recombinant DNA reagent |
AncA* (plasmid) | 10.1371/journal.pbio.1001994 | n/a | |
| Recombinant DNA reagent |
AncB* (plasmid) | 10.1371/journal.pbio.1001994 | n/a | |
| Recombinant DNA reagent |
AncC* (plasmid) | 10.1371/journal.pbio.1001994 | n/a | |
| Recombinant DNA reagent |
AncD* (plasmid) | 10.1371/journal.pbio.1001994 | n/a | |
| Recombinant DNA reagent |
ecRNH* (plasmid) | 10.1002/pro.5560030906 | n/a | |
| Recombinant DNA reagent |
ecRNH* A55G (plasmid) |
this paper | n/a | A55G point mutant of ecRNH* |
| Recombinant DNA reagent |
ecRNH* D108L (plasmid) |
this paper | n/a | D108L point mutant of ecRNH* |
| Commercial assay or kit |
QuikChange Site-Directed Mutagenesis Kit |
Agilent | 200519 | |
| Commercial assay or kit |
Amicon Ultra Protein Concentrator | Millipore | UFC900324 | |
| Chemical compound, drug |
Glycine | JT Baker | 4059–06 | |
| Chemical compound, drug |
Urea | IBI Scientific |
IB72064 | |
| Chemical compound, drug |
Sodium Acetate |
VWR Life Sciences |
0530–1 KG | |
| Chemical compound, drug |
Deuterium Oxide |
Sigma Aldrich |
151882–100G | |
| Chemical compound, drug |
Trifluoroacetic Acid |
Sigma Aldrich |
302031–100 ML | |
| Chemical compound, drug |
Acetonitrile | Thermo Fisher |
85188 | |
| Software, algorithm |
ExMS | 10.1007/s13361-011-0236-3 | n/a | |
| Software, algorithm |
HDSite | 10.1073/pnas.1315532110 | n/a | |
| Software, algorithm |
HDExaminer | Sierra Analytics |
n/a | |
| Software, algorithm |
Illustrator | Adobe | n/a | |
| Software, algorithm |
GraphPad Prism |
GraphPad Software, Inc. |
n/a | |
| Other | POROS 20 R2 Beads |
Thermo Fisher |
1112810 | |
| Other | Microbore column |
Upchurch Scientific |
C-128 | |
| Other | Reverse-phase analytical column |
Thermo Fisher |
72205–050565 | |
| Other | POROS 20 AL Beads |
Thermo Fisher |
1602810 | |
| Peptide, recombinant protein |
Pepsin (porcine) |
Sigma Aldrich | P6887 | |
| Peptide, recombinant protein |
Fungal Protease Type-XIII |
Sigma Aldrich | P2143 | |
| Peptide, recombinant protein |
Pfu polymerase | Agilent | 600353 |