Table 2 –
Evaluation of cell cycle phase at different time-points.
| Time of treatment (h) | Treatment Group | Cell count (%) | |||
|---|---|---|---|---|---|
| G0/G1 phase | S phase | G2 phase | Necrosis | ||
| 24 | DMSO | 56.93 ± 12.59 | 12.25 ± 5.02 | 25.80 ± 10.80 | 3.52 ± 3.17 |
| Carb | 52.33 ± 12.07 | 18.15 ± 2.52 | 20.83 ± 0.33 | 6.62 ± 7.83 | |
| Carb UV | 43.52 ± 12.52 | 23.13 ± 10.18 | 26.30 ± 4.67 | 3.99 ± 4.01 | |
| DMSO UV | 60.63 ± 0.66 | 9.58 ± 0.49 | 25.52 ± 3.18 | 2.99 ± 2.83 | |
| 48 | DMSO | 56.72 ± 5.83 | 11.06 ± 2.77 | 29.35 ± 5.03 | 1.96 ± 1.38 |
| Carb | 49.68 ± 5.35 | 16.33 ± 2.02* | 28.85 ± 7.66 | 4.09 ± 5.73 | |
| Carb UV | 45.76 ± 7.88* | 16.65 ± 4.27* | 33.70 ± 8.16 | 3.11 ± 3.58 | |
| DMSO UV | 52.99 ± 6.36 | 11.77 ± 1.68 | 31.41 ± 6.86 | 2.81 ± 3.09 | |
| 72 | DMSO | 61.12 ± 3.36 | 10.35 ± 3.85 | 24.52 ± 5.42 | 2.42 ± 2.00 |
| Carb | 54.14 ± 4.00 | 17.20 ± 6.24 | 25.56 ± 3.79 | 1.59 ± 0.25 | |
| Carb UV | 49.31 ± 6.90* | 19.38 ± 8.26 | 27.03 ± 2.39 | 2.44 ± 1.14 | |
| DMSO UV | 59.40 ± 1.75 | 11.64 ± 2.72 | 24.83 ± 1.35 | 2.32 ± 2.36 | |
Data from 24h, 48h and 72h were generated in experimental duplicates, quintuplicates and triplicates, respectively. No difference was observed between non-treated control group and the vehicle control (data not shown). 48h and 72h data were analyzed by ANOVA followed by Dunnett’s test to evaluate differences between treated groups and the vehicle control group (*p<0.05). Results are expressed as mean ± standard deviation. DMSO UV – treated with DMSO 0.07% and 375 mJ/cm2 solar radiation; Carb UV – treated with carbaryl 100 μM and 375 mJ/cm2 solar radiation; Carb – treated with carbaryl 100 μM; DMSO – treated with DMSO 0.07%.