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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Cancer Lett. 2018 May 8;431:1–10. doi: 10.1016/j.canlet.2018.04.044

Figure 1. Kaiso binds to CpG sites directly regulating miR-200a, miR-200b, miR-200c, miR-141 and miR-429 expression.

Figure 1.

(A) in silico analysis of Kaiso binding sites on the promoters of each miRNA in 200 family. (B) Chromatin immunoprecipitation products were analyzed by qRT-PCR specific miR-200a, miR-200b, miR-429 core promoter primers (−321 to +120) to amplify the methylated region in PC3 cells in the presence or absence of 5-aza. (C) Chromatin immunoprecipitation products were analyzed by qRT-PCR miR-200c and miR-141 core promoter primers (246 to −169) to amplify the methylated region in PC3 cells in the presence or absence of 5-aza Mouse IgG as a negative control. Input DNA served as a positive control. All data presented are the means of three independent experiments, performed in triplicate ± S. E. ***P < 0.001 is significant