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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Cancer Lett. 2018 May 8;431:1–10. doi: 10.1016/j.canlet.2018.04.044

Figure 3. Kaiso regulates miR-200c through EGFR activity.

Figure 3.

A. Relative expression levels of miR-200c expression levels were measured by qRT-PCR in a panel of prostate cancer cells lines shows that miR-200c is significantly lower in DU-145 cells that overexpress EGFR compared to parental cell lines. B. miR-200c expression was measured by qRT-PCR in DU-145, DU-145 EGFR, and PC-3 cells were treated with 10ng/mL and 50ng/mL of EGF, as well as an EGFR specific kinase inhibitor, PD153035. C. miR-200c expression was measured by in sh-Kaiso DU-145 and PC-3 cells in the presence or absence of 10ng/mL EGF. D. Stable LNCaP Kaiso overexpression (LNCaP-O) and LNCaP SCR-control cell were measured for Kaiso, EGFR, p-EGFR, and ZEB1 by immunoblot with β-actin as loading control. E. miR-200a, miR-200b, miR-429, miR-200c and miR-141 were measured using RT-PCR in LNCaP Kaiso overexpressing and LNCaP scr-control cells. All data presented are the means of three independent experiments, performed in triplicate ± S. E * P < 0.05 is significant. **P < 0.01 is significant. ***P < 0.001 is significant.