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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Cancer Lett. 2018 May 8;431:1–10. doi: 10.1016/j.canlet.2018.04.044

Figure 4. miR-200c Re-expression Reverses EMT.

Figure 4.

A. Two stably transfected clones 4 and 10 in PC-3 cells were measured for qRT-PCR for miR-200c expression compared to cells transfected with SCR-control and LNCaP cells. B. PC-3 miR-200c clone 10 was probed for Kaiso, Zeb 1 and Zeb 2 expression by qRT-PCR and compared to SCR-control and LNCaP cells. C. Kaiso, EGFR, p-EGFR, and ZEB1 were measured by immunoblot in miR-200 clone PC-3 cells, SCR-control and LNCaP cells. Immunofluorescence using Anti-Kaiso antibody (yellow) expression in miR-200c PC-3 clone compared to PC-3 wild type and scramble control cells. Blue color =DAPI nuclear stain. All data presented are the means of three independent experiments, performed in triplicate ± S. E * P < 0.05 is significant. **P < 0.01 is significant. ***P < 0.001 is significant