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. 2018 Jul 30;16:65–75. doi: 10.1016/j.molmet.2018.07.011

Figure 2.

Figure 2

Activation of Insl5-cell enriched GPCRs triggers Ca2+-responses. (A) Relative expression of a subset of colonic L-cell GPRCs in FACS GFP+ve and GFP−ve cells following in vivo doxycycline induction (n = 3 mice). Bars represent mean 2ΔCT + SEM compared to β-actin. Statistical significance was assessed through ratio paired t-tests applied to 2ΔCT. ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. (B) Representative trace from a single calcium imaging experiment. (C,D) Intracellular calcium responses of INSL5-producing L-cells to positive controls angiotensin II (10 nM) and bombesin (100 nM) (C) and to agonists of CASR (5 mg/ml peptones), FFA1 (1 μM AM-1638), AVPR1B (10 nM AVP) and FFA2 (2 mM butyrate) (D).