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. Author manuscript; available in PMC: 2019 Feb 20.
Published in final edited form as: Nat Microbiol. 2018 Aug 20;3(10):1109–1114. doi: 10.1038/s41564-018-0221-8

Figure 4: Chemical complementation restores MNoV infection and a CD300lf conformation in Sptlc2 deficient cells.

Figure 4:

(a) MNoVCW3 infection of indicated cell lines either treated with DMSO or C2 ceramide. Infection was measured by FACS for intracellular production of VP1. Data are shown as means ± SEM from three independent experiments and data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. *p<0.05, ***p<0.001, ns = not significant.

(b) Histogram of a representative histogram from three independent experiments looking at the CD300lf conformation-dependent antibody TX70 ability to stain indicated cells treated with either DMSO or C2 ceramide.

(c) Quantification of mean fluorescence intensity (MFI) of α-CD300lf antibody TX70 from three independent experiments. MFI within an experiment was normalized to BV2 + DMSO. Data are shown as means ± SEM from three independent experiments and data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. *p<0.05, ***p<0.001, ns = not significant.