Fig. 4.

Fungicidal activity of hemolymph is Bom-dependent. Hemolymph activity was assayed for 4 sets of flies: w¹¹¹⁸ (wild-type), Bom∆^55C, and Bom∆^55C carrying either a 55C-Right (four-gene) or Bom065 (single-gene) construct. All flies had been pricked 1 day earlier with heat-killed M. luteus to induce Toll-mediated gene expression. Fungicidal activity was assayed by incubating cell-free hemolymph with C. glabrata for 1 h at room temperature (RT) or for 24 h at 4°C before spreading the mixture onto a yeast (YPD) plate. Percent killing was calculated by comparing the colony count for each sample to that for yeast mixed with a w¹¹¹⁸ uninduced hemolymph control. Experiments were performed in triplicate; error bars represent standard error of the mean. One-way ANOVA followed by Tukey's test was performed for each incubation condition. Significance is shown relative to the w¹¹¹⁸ uninduced control (⚫⚫⚫ and ◆◆◆ = p < 0.0001; ns, not significant, p > 0.05).