Figure 1. IL-1R1 is dispensable for protective immunity against MDR Mtb strain, W_7642.

B6 (n=10) and gene deficient mice (Myd88^−/− HN878 n=3, W_7642 n=6; Ifngr^−/− HN878 n=5, W_7642 n=6; Tnfr1^−/− n=4; Nos2^−/− n=5; Il1r1^−/− HN878 n=9, W_7642 n=5; Ifnar^−/− n=5; Il10^−/− n=5) were aerosol infected with 100 CFU Mtb HN878 or W_7642. Lung bacterial burden was determined on 30 dpi (a) or at defined time points (b, B6 n=5, Il1r1^−/− n=9 except W_7642 D15, D20 Il1r1^−/− n=5). HN878-infected Il1r1^−/− mice were sacrificed on 30 dpi due to severe TB disease (b). On 30 dpi, formalin-fixed, paraffin embedded (FFPE) lung sections from B6 and Il1r1^−/− mice were stained with H&E and inflammatory area was measured. Micrographs are representative images - 5x magnification (c, HN878 B6 n=9, HN878 Il1r1^−/− n=8, W_7642 n=7). Total number of lung neutrophils, monocytes, and recruited macrophages were determined on 30 dpi using flow cytometry (d, B6 n=5, Il1r1^−/− n=4, UI B6 n=4). Cytokine and chemokine protein levels in lung homogenates were measured in B6 and Il1r1^−/− mice at 30 dpi (e, n=5). UI-uninfected. (a) 1-way ANOVA with Tukey’s post-test, (b,d,e) 2-way ANOVA with Bonferroni post-test, (c) two tailed Student’s t-test. The data points represent the mean (±SD) of values. *p≤0.05, **p≤0.01, ***p≤0.001, ns-not significant (p>0.05).