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. 2018 Jun 20;16:126–138. doi: 10.1016/j.molmet.2018.06.012

Figure 3.

Figure 3

Effect of Cvs and long-term effects of Cvs intervention on muscle metabolism.Ex vivo glucose uptake under basal and insulin-stimulated conditions in intact isolated EDL muscle from Cvs (A) and three months post Cvs (Cvs3m) mice (B) compared to the age-matched controls (Ctrl and Ctrl3m, respectively). Representative western blot of insulin stimulated pAKT and pAMPK from EDL muscle obtained from 3 Cvs3m and Ctrl3m mice collected after ex vivo glucose uptake assay (C) and representative densitometric analysis (D, E). Ex vivo FAO in intact isolated Soleus muscle under basal and AICAR-stimulated conditions (F, I), ß-oxidation-linked respiration (G, J), TCA-linked respiration (H, K) in Soleus muscle. Data presented as mean ± s.e.m; two-tailed unpaired Student's t-test or by Two-Way ANOVA followed by Bonferroni post hoc (A-B, D-F and I) *p < 0.05, **p < 0.01, ***p < 0.001, FAO and mitochondrial respiration n = 7–18, n = 6–16 in EDL muscle glucose uptake and n = 3 for western blot analysis.