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. 2018 Jun 20;16:126–138. doi: 10.1016/j.molmet.2018.06.012

Figure 4.

Figure 4

Effect of Cvs and long-term effects of Cvs intervention on fat metabolism.Ex vivo glucose uptake under basal and insulin-stimulated conditions in primary adipose cells from Cvs (A) and three months post Cvs (Cvs3m) mice (B) compared to the age-matched controls (Ctrl and Ctrl3m, respectively) n = 5–6. Representative white adipose tissue western blot of insulin stimulated pAKT and pAMPK (C) obtained from Cvs3m and Ctrl3m mice injected with insulin for 10 min and representative densitometric analysis of (D and E) n = 6. Representative western blot and densitometry analysis of GLUT1 (F) n = 6, mRNA expression analysis of Slc2a1 (Glut1) (G), Slc2a4 (Glut4) (H) n = 6 and browning and thermogenic marker from eWAT following 3-month post CVS and control mice (I) n = 6. Representative western blot and densitometry analysis of UCP1 (J) n = 6 and mRNA analysis of browning and thermogenic marker from BAT from Cvs3m and Ctrl3m mice (K) n = 7–10. Values are means ± s.e.m. Statistical analyses were done by two-tailed unpaired Student's t-test (F–K) or by Two-Way ANOVA followed by Bonferroni post hoc (A-B, D-E). *p < 0.05, **p < 0.01, ***p < 0.001.