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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Mol Microbiol. 2018 Jul 31;109(3):345–364. doi: 10.1111/mmi.13989

Figure 8. Fluorescence Polarization experiments with labeled XrpA-N1.

Figure 8

(A) Selectivity of FITC-labeled XrpA-N1 probe to ComR (black) over other S. mutans purified recombinant proteins SppA (blue) and CcpA (red). Fluorescent polarization (FP) curves were generated using increasing concentrations of purified proteins binding to 10 nM of fluorescently labeled XrpA-N1 probe in the presence of 10 μM sXIP. (B) Increasing concentrations of synthetic unlabeled XrpA-N1 peptide were assessed for their ability to compete with FITC-labeled XrpA-N1 for their binding to increasing concentrations of purified ComR. (C). Binding of 10 nM FITC-labeled XrpA-N1 peptide to increasing concentrations of purified ComR, with and without 10 μM sXIP addition. (D) Binding of 10 nM FITC-labeled XrpA-N1 peptide to increasing concentrations of purified ComR in addition to increasing concentrations of sXIP peptide. Data shown for all graphs are averages of three independent experiments.