Fig. 3.

Viability of neutrophils under aerobic and anaerobic conditions and co-cultivation with stem cells and epithelial cells. Neutrophils were cultivated for 24 h in DMEM under aerobic or anaerobic conditions. The co-cultivation with cells was performed at a ratio of 1: 10 PMNs. Neutrophil apoptosis was analyzed via Annexin V/7-AAD staining and flow cytometry analysis. The percentage of cells without signs of apoptosis was plotted as the percentage in relation to the total cell numbers. Comparison between aerobic and anaerobic conditions in the presence or absence of hDFSCs (a) and co-culture of neutrophils with hBMSCs and Ca9–22 in anaerobic conditions (b) are demonstrated. The results are displayed as the median; * p < 0.05, ** p < 0.01 were considered significant (Mann-Whitney U test), n ≥ 4 (each data point represents 1 independent biological replicate).