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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Curr Protoc Cell Biol. 2018 Aug 2;80(1):e56. doi: 10.1002/cpcb.56
Monomer solution (“Stock X”) Stock solution concentration (g/100 mL solution) Amount (mL) Final concentration (g/100 mL solution)
Sodium acrylate 38 (33 wt% due to higher density) 2.25 8.6
Acrylamide 50 0.5 2.5
N,N′-Methylenebisacrylamide 2 0.75 0.15
Sodium chloride 29.2 (5M) 4 11.7
PBS 10x stock 10x 1 1x
Water 0.9
Total 9.4