Digestion buffer	Amount	Final concentration (/100 mL solution)
Triton-X	2.50 g	0.50 g
EDTA	0.146 g	0.027 g
Tris (1M) aqueous solution, pH 8	25 mL	5 mL
NaCl	23.38 g	4.67 g
Water	Add up to a total volume of 500 mL
Proteinase K (800 units/mL)	1:100 dilution	800 units (= 8 units/mL)
Total	500 mL