Fig. 1.

Crystal structure of the Drosophila PR-DUB complex. a Schematic representing domain structure of human and Drosophila PR-DUB components. Sequence identity between the UCH and ULD domains of Calypso and BAP1, and between the Deubad domains of ASX and ASXL1 is indicated. FL, full-length; UCH, ubiquitin C-terminal hydrolase; ULD, UCH37-like domain; ASX, additional sex combs; ASXL1, ASX-like 1; PHD, plant homeodomain (see Supplementary Fig. 1a). b Structure of the Calypso–ASX complex (PDB code: 6CGA). The UCH and ULD domains of Calypso are coloured orange and blue, respectively. The Deubad domain of ASX is coloured green. Black arrows indicate the position of the active site cysteine residue in the respective UCH domains. Deubad (DEU), deubiquitinase adaptor domain (see Supplementary Table 1). c A model of the Calypso~Ub–ASX complex (based on PDB 4UEL; ref. ¹⁶). The Calypso–ASX structure is shown in surface representation and colour coded as in b, while the modelled ubiquitin and the ASX Deubad domain in chain A are shown as cartoon and coloured grey and green respectively. Ub, ubiquitin