Table 1.
List of sampling dates, sites sampled on each date, as well as mean data for all parameters measure with standard deviations in parentheses.
| Date | Sample Type | Number of Samples | Sites Sampled | log10 E. coli (CFU 100 mL−1) | Caffeine (ng L−1) | Carbamazepine (ng L−1) | Codeine (ng L−1) | Cotinine (ng L−1) | Acetaminophen (ng L−1) | qPCR | eDNA Sequences | % Agreement Between qPCR and eDNA for Human Detection | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| log10 Human (CN 100 mL−1) | % Human Positive | log10 Gull (CN 100 mL−1) | Human | % Human Positive | Mammal | Frog | Bird | Fish | |||||||||||
| 6/27/2013 | Creek | 7 | 80002, 80006, BURN, DDAS, DERY, EGLI, WCRK | 2.26 (0.54) | 324.34 (355.05) | 0.72 (0.10) | 0 | 51.27 (7.95) | 6.46 (6.27) | 0 | 0 | 0 | 26.14 (28.42) | 66.67 | 0 | 0 | 0 | 110.14 (92.73) | 42.86 |
| 7/4/2013 | Creek | 10 | 80002, 80006, BURN, DDAS, DERY, EGLI, MALL, RB, SWAY, WCRK | 2.95 (0.41) | 435.26 (363.67) | 1.05 (0.26) | 0 | 34.34 (15.04) | 53.57 (45.75) | 1.05 (1.77) | 30 | 0.03 (0.10) | 36.90 (101.01) | 60.00 | 1.0 (2.83) | 0 | 1.80 (4.47) | 64.50 (50.43) | 50 |
| 7/9/2013 | Creek | 9 | 80002, 80006, BURN, EGLI, MALL, RB, SWAY, S1, WCRK | 3.92 (0.11) | 130.73 (86.53) | 0.66 (0.86) | 0 | 12.41 (8.75) | 19.40 (24.44) | 1.14 (1.79) | 33.33 | −0.06 (0.18) | 2.56 (3.88) | 55.56 | 4.78 (4.99) | 1.33 (1.66) | 1.22 (1.09) | 8.44 (6.0) | 55.56 |
| 6/27/2013 | Beach | 7 | E.1, E.1.5, E.2, W.1, W.2, W.3 | 6.24 (0.99) | NT | NT | NT | NT | NT | 0 | 0 | 0 | 9.67 (21.48) | 28.57 | 0 | 0 | 0.33 (0.76) | 15.50 (12.10) | 71.42 |
| 7/4/2013 | Beach | 1 | W.3 | 3.24 (0) | NT | NT | NT | NT | NT | 0 | 0 | 0 | 3 (0) | 100.00 | 0 | 0 | 0 | 21 (0) | 0 |
| 7/9/2013 | Beach | 9 | E.1, E.1.5, E.2, W.1, W.2, W.3 | 3.79 (0.58) | NT | NT | NT | NT | NT | 2.76 (1.77) | 77.75 | 0.45 (1.02) | 2.56 (6.21) | 44.44 | 0.67 (1.66) | 0 | 1.56 (2.19) | 13.11 (18.0) | 44.44 |
| 6/27/2013 | Outfall | 4 | COURTOF, DDASOF, GARNOF, SWAYOF | 3.16 (0.50) | 260.55 (408.91) | 24.18 (44.27) | 2.12 (4.23) | 23.48 (10.90) | 39.23 (40.14) | 0.78 (0.155) | 25 | 0 | 225.75 (440.24) | 50.00 | 61.75 (122.17) | 0 | 1.0 (2.0) | 89.75 (128.17) | 75 |
| 7/4/2013 | Outfall | 3 | DDASOF, GARNOF, SWAYOF | 3.90 (0.15) | 7886.67 (10925.12) | 1.21 (0.69) | 0 | 173.37 (87.0) | 1287.33 (1447.25) | 2.55 (2.34) | 66.67 | 1.51 (0.77) | 30.33 (37.03) | 100.00 | 70.33 (108.83) | 0.67 (1.15) | 180.67 (153.24) | 4.67 (2.52) | 66.67 |
| 7/9/2013 | Outfall | 2 | DDASOF, EGLIOF | 3.73 (0.36) | 253.40 (217.22) | 0.53 (0.65) | 0 | 42.20 (44.55) | 102.63 (134.88) | 3.20 (0.46) | 100 | 0 | 7.50 (7.78) | 100.00 | 0.50 (0.71) | 0 | 0 | 7.0 (9.90) | 100 |
The percentage of samples positive for the human MST method and eDNA metabarcoding are also presented.
NT denotes that the parameter was not tested.