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. 2018 Sep 26;8:14380. doi: 10.1038/s41598-018-32706-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Using microelectrodes in microwells to assay protein from cells. (a) The image, acquired by TIRF, shows an anti-GFP antibody spot printed onto a PEI- and microelectrode-coated coverslip and aligned into an individual well of the microwell array. Image shows GFP captured by the antibody spot (within dotted red line). Scale bar length is 25 μm. (b) The number of cells per well follows a Poisson distribution. The antibody spot in each microwell captures fluorescent GFP protein from the electrically lysed cells. The results show that, as expected, the GFP signal increases with increasing GFP-expressing cell occupancy.