Fig. 1.

Analysis of UBL3 as a post-translational modification factor. a UBL3-dependent posttranslational modification was detected by immunoprecipitation (IP) with anti-Flag antibodies from MDA-MB-231 cells transfected with Flag-UBL3, followed by western blotting with UBL3 antiserum (right panel). b The tissue extracts from the cerebral cortex of WT and Ubl3 KO mice were immunoprecipitated with anti-UBL3 antibodies. c, f Schematic structures of Flag-tagged wild-type or mutant UBL3. d, g Detection of UBL3 modification in these cells. IP products were boiled without 2-mercaptoethanol (βME-). A portion of the samples was treated with 2-mercaptoethanol (βME+). e, h Subcellular localisation of UBL3 in MDA-MB-231 cells transfected with Flag-UBL3 (wild-type and mutants). Twenty μg per lane