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. 2018 Sep 26;9:3936. doi: 10.1038/s41467-018-06197-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — EGFP and biotinylated protein tagged by UBL3 are sorted to the sEVs. EGFP-UBL3 (left panel) and biotinylated protein tagged by UBL3 (right panel), but not EGFP-UBL3C113/114A or biotinylated protein tagged by UBL3C113/114A were preferentially accumulated in the sEVs of the cell culture media. After the western blot analysis, gels were stained with SYPRO Ruby (lower panel). Before loading, the samples were boiled with βME. The same amounts of protein were loaded on the gels (Cell Lysate, 20 μg per lane; sEVs, 1 μg per lane)