Fig. 1. Danshen improved survival of patients with osteosarcoma and TIIA inhibited anchorage-independent growth in vitro and osteosarcoma growth in vivo.

a Kaplan–Meier survival curves for osteosarcoma patients (n = 22; P = 0.005) with or without Danshen treatment. b Soft agar colony formation of osteosarcoma cell lines (143B and MG63) treated with various concentrations TIIA for 3 weeks. Histogram represented average number of colonies counted (in six microfields). Results were representative of three independent experiments. Scale bar: 500 μm. c–f 143B cell-derived tumors were developed in NOD/SCID mice and treated with TIIA or vehicle. Representative tumor images were photographed (c). Tumor growth was monitored by histological staining (H&E) (d) and measuring the tumor volume (e) and weight (f) for 45 days (n = 15 mice/group; *P < 0.05 compared with vehicle controls). The results were expressed as the means ± SD