Table 2.
Mutation efficiency of the two different CRISPR/Cas9 vectors and vector delivery systems.
| Colonies in total | Screened colonies | Edited colonies | Percentage edited colonies | |
|---|---|---|---|---|
| Myb PAM 1 | ||||
| Biolistic | 19 | 12 | 6 | 50% |
| Bacterial Conjugation | 900 + | 12 | 4 | 33% |
| Myb PAM 2 | ||||
| Biolistic | 15 | 12 | 4 | 33% |
| Bacterial Conjugation | 500+ | 12 | 3 | 25% |
The presented data is from one-month-old primary colonies resulting from the introduction of the pKS diaCas9_sgRNA vector using biolistic bombardment, and three-months-old (re-plated once per month) colonies transformed by delivery of the pPtPuc3m diaCas9_sgRNA vector by bacterial conjugation. Edited colonies were identified by HRM and targeted gene mutations were confirmed by sequencing.