Fig. 2.

Talpid3 regulates centriole maturation and vesicle docking through distinct regions. a Centrosomal and ciliary defects of Talpid3^−/− cells were rescued by infection with lentiviruses expressing different Myc-tagged Talpid3 constructs. Cells were serum-starved for 48 h and examined by IF with antibodies against indicated antibodies. Coiled-coil domain and CRISPR-targeted region of Talpid3^−/− cells are shown in orange and red (scissors), respectively. Specific truncation proteins are indicated with an asterisk. The fragment spanning residues 400–700 is not recognized by Talpid3 antibodies and thus was detected by Myc tag, shown in Supplementary Figure 1b. b Stable Talpid3^−/− cell lines expressing Talpid3 truncations were transduced with lentiviruses expressing SmoM2-GFP. Two days after transduction, cells were serum-starved for 6 h and stained with indicated antibodies. Cumulative data from three independent experiments are shown. For each group, a minimum of 100 cells/experiment was averaged. All data are presented as mean ± SD. *p < 0.05 (unpaired t-test). Scale bar = 2 μm