Fig. 5.

Asymmetric localization of DC-enriched proteins is required for proper localization of OFD1. a WT RPE1 cells were transduced with lentiviruses expressing Myc-PACT–CEP120, Myc-PACT–Centrobin, or Myc-PACT-Neurl4, as indicated. Two days after transduction, cells were serum-starved for 48 h and examined by IF with indicated antibodies. b Control, C2CD3^−/−, and Talpid3^−/− cells were transfected with siRNAs against Centrobin. Two days after transfection, cells were serum-starved for 24 h and were visualized with indicated antibodies. c OFD1 recruitment defect in Talpid3^−/− cells was rescued by infection with lentiviruses expressing the Talpid3–C2CD3 fusion protein described in Fig. 3e. Cells were examined by IF after 48 h of serum starvation using indicated antibodies. d Model indicating a centriole maturation regulatory mechanism. Cumulative data from three independent experiments are shown. For each group, a minimum of 100 cells/experiment was averaged. All data are presented as mean ± SD. *p < 0.05 (unpaired t-test). Scale bars = 2 μm