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. 2018 Sep 26;8:14412. doi: 10.1038/s41598-018-32819-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pattern of conserved motifs of the NRAMP protein and their association with phylogenetic relationship. (a) Conserved motifs of NRAMP proteins were identified in 171 sequences from selected species of Bacteria, algae, moss, spike moss (Selaginella) and angiosperms using the MEME search tool. Consensus and discriminative motifs are indicated by rectangular box and wedge shapes, respectively. The motif matches are shown with a cut-off p-value less than 0.00001. (b) Multiple alignment of coding nucleotide sequences of motif1 and motif9 was determined based on their corresponding amino acid translations using TranslatorX server. (c) Sequence logo of the motif1 and motif9 was generated by the WebLogo application. Four conserved residues of the substrate biding site are shaded grey.