Table 1.
Haematological parameters and platelet surface analysis of glycoprotein receptor levels.
| Parameter | WT | KO | P value | ||||
|---|---|---|---|---|---|---|---|
| Mean | SEM | N | Mean | SEM | N | ||
| WBC (103/mm3) | 6.04 | 0.52 | 9 | 5.651 | 0.55 | 9 | 0.617 |
| RBC (106/mm3) | 11.28 | 0.58 | 9 | 11.06 | 0.38 | 9 | 0.749 |
| Plt (103/mm3) | 926 | 61 | 9 | 896 | 36 | 9 | 0.686 |
| MPV (µm3) | 5.51 | 0.08 | 9 | 5.65 | 0.08 | 9 | 0.245 |
| Plateletcrit (%) | 0.414 | 0.038 | 9 | 0.409 | 0.021 | 9 | 0.906 |
| GPIIb (MFI) | 8507 | 92 | 5 | 8558 | 77 | 5 | 0.678 |
| GPIbα (MFI) | 2290 | 292 | 5 | 2203 | 265 | 5 | 0.829 |
| GPVI (MFI) | 883 | 25 | 5 | 862 | 29 | 5 | 0.586 |
Whole blood from WT and KO mice drawn in 4% citrate was analysed on a Horiba Pentra ES60 haematological analyser, with cell counts corrected for dilution in citrate. WBC, white blood cell; RBC, red blood cell; Plt, platelet; MPV, mean platelet volume; PCT, plateletcrit. Washed platelets (2 × 107/mL) from WT and KO platelets were analysed for surface expression of GPIIb, GPIbα and GPVI by flow cytometry. Median fluorescent intensity (MFI) values are reported. Data are mean ± s.e.m and the indicated number (N) of independent experiments. P values were determined by unpaired student’s t-test (two-tailed).