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. 2018 Sep 26;8:14427. doi: 10.1038/s41598-018-32327-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ant venom promotes eosinophil recruitment to peritoneal cavity. Mice were previously injected (sensitized: S) with saline (Sal), OVA + AlOH₃ (SOACO), ant venom (10 μg = S10; 100 μg = S100), or OVA + 10 μg ant venom (SOVCO). After 2 weeks, mice were submitted to an intraperitoneal challenge (Challenge: C) with saline, OVA (CO), or 10 μg ant venom extract (C10). Twenty-four hours after the challenge, the peritoneal cells were harvested and the eosinophils were counted. Measurements are expressed as the percentage of eosinophils among the peritoneal cell population. Boxplot of pooled internal replicates from three independent experiments, where the vertical lines are upper and lower limits, and the internal line are median values. Different letters indicate statistical significance (p < 0.05) between groups as compared by Kruskal-Wallis followed by Dunn’s Multiple Comparisons Test.