. 2018 Sep 26;9:3935. doi: 10.1038/s41467-018-06403-x

Table 4.

The selectivity of APPI-3M variants (normalized to the unmodified APPI-3M) toward KLK6 versus the three other proteases

Mutant	vs. mesotrypsin	vs. anionic trypsin	vs. cationic trypsin	Calculated KLK6 total selectivity^a	Expected KLK6 total selectivity^b
Unmodified APPI-3M	1	1	1	1	—
T11V/G17R	111.10	8.94	2.32	2304.13	242.45
T11S/G17R	31.62	3.87	0.94	115.53	32.33
T11S	2.14	0.30	0.12	0.08	—
T11V	0.64	1.58	0.60	0.60	—
G17R	32.26	7.42	1.69	404.09	—

^a $C a l c u l a t e d s e l e c t i v i t y = \frac{\frac{K i_{W T} f o r K L K 6}{K i_{m u t a n t} f o r K L K 6}}{\frac{K i_{W T} f o r o t h e r p r o t e a s e i n t h e p a i r}{K i_{m u t a n t} f o r o t h e r p r o t e a s e i n t h e p a i r}}$

^bExpected selectivity of double-mutant AB = calculated selectivity of A × calculated selectivity of B