Fig. 2.

MinDE regulate a variety of peripheral membrane proteins to different extents. a Overview of the model peripheral membrane proteins employed. All amphipathic helices were fused to mCherry at their endogenous terminus. b Representative images of the MinDE wave (upper panel) and the anticorrelated mCh-MTS wave with two different brightness settings (middle and lower panels) on the membrane (1 µM mCh-MTS, 1 µM MinD (30% EGFP-MinD), 1 µM MinE). All images in one row were acquired and displayed using the same instrumental settings. Fluorescence intensity line plots of the corresponding images (EGFP-MinD fluorescence in green, mCh-MTS fluorescence in magenta) show the difference in the extent of the spatial regulation (lowest panel). c mCh-MTS constructs with a C-terminal amphipathic helix exhibit highest contrast. Box plot of the contrast of mCh-MTS constructs, lines are median, box limits are quartiles 1 and 3, whiskers are 1.5× interquartile range (IQR) and points are outliers. d mCh-MTS intensity in the MinDE maximum (${\mathit{I}}_{\max \left(\mathrm{MinD}\right)}^{\mathrm{mCh-MTS}}$) normalized to His-mCh and corrected for the fluorescent protein fraction. Each data point (square, sphere, triangle) corresponds to one independent experiment (exp 1–3) and was generated from at least one tile scan (7 by 7) in one sample chamber (number of images N_His-mCh = 343, N_{MTS(1×MreB)-mCh} = 294, N_{mCh-MTS(FtsA)} = 490, N_{MTS(FtsY)-mCh} = 392, N_mCh-MTS(BsD) = 390, N_{MTS(2×MreB)-mCh} = 265). Cross and error bars represent the mean value and standard deviation of the three independent experiments. Scale bars: 50 µm