Fig. 6.

MinDE spatiotemporally regulate membrane-anchored DNA. a MinDE self-organization can regulate short membrane-anchored DNA fragments. Representative images and kymograph of a time-series of MinDE self-organization in the presence of 30 bp P1 dsDNA bound to the membrane by a cholesterol anchor (1 µM MinD (30% EGFP-MinD), 1 µM MinE, 10 nM TEG-cholesterol-dsP1). b Representative images and kymograph of a time-series of MinDE self-organization spatiotemporally regulating 300 bp long dsDNA bound to lipid-anchored streptavidin (1 µM MinD (30% EGFP-MinD), 1 µM MinE, 300 bp lambda DNA, streptavidin). c Representative images and kymograph of a time-series of MinDE self-organization spatiotemporally regulating 2000 bp long dsDNA bound to lipid-anchored streptavidin. All experiments were performed independently two (c) or three (a, b) times under similar conditions. Scale bars: 50 µm