Fig. 7.

MinDE-driven dynamics of model membrane proteins in vitro suggest that MinDE form a propagating diffusion barrier. a Representative images and kymographs of colliding MinDE waves in the presence of mCh-MTS(BsD) and lipid-anchored streptavidin bound to biotinylated lipids (1 µM MinD (30% EGFP-MinD), 1 µM MinE, 1 µM mCh-MTS(BsD) or streptavidin-Alexa647). Scale bars: 50 µm. b Schematic of the underlying protein behavior resulting in spatiotemporal regulation of model peripheral and membrane-anchored proteins. While mCh-MTS and MinDE can also attach and detach to and from the membrane, streptavidin can only diffuse laterally on the membrane. Schematic density profiles and protein localization on the membrane (magenta: mCh-MTS, green: MinD, orange: MinE, cyan: lipid-anchored streptavidin). The MinDE wave propagates directionally, even if individual proteins show a random movement on the membrane. Both model peripheral and membrane-anchored proteins show a wave propagation in the direction of the MinDE wave. mCh-MTS while more abundant in the MinDE minima covers the membrane homogenously. In contrast the resulting secondary wave of streptavidin shows an inhomogeneous profile and results in a net transport of the membrane-anchored protein