Fig. 1. Ensuring adequate transfection in the mouse retina using an AAV2 CAG vector system.

a Schematic of the procedure performed. b–e Expression of GFP throughout the retina using various titres and volumes of AAV2 GFP (n = 4/group). f A tile scan of a representative retinal wholemount 3 weeks after injection of 2 µl AAV2 GFP at 1 × 10¹⁰ vector particles/eye. fi GFP expression is localized to the inner retina with transduction of predominantly RGCs and some amacrine/Müller glia cells. fii Magnification of the wholemount shows co-localization between GFP and Brn3A+ RGCs. g–gii GFP expression can also be seen transported anterogradely down the optic nerve in both proximal (g) and distal (gi, ii) locations