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. 2018 Sep 20;8:382. doi: 10.3389/fonc.2018.00382

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Copyright © 2018 Zorofchian, Lu, Zhu, Duose, Windham, Esquenazi and Ballester.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Droplet digital PCR results from examination of the CNS tissue and the CSF-ctDNA for MYD88 mutations. Quadrant statistics scatter plot (lower left quadrant shows negative cluster; lower right quadrant shows wild-type cluster; upper left quadrant shows mutant cluster; upper right quadrant shows double positive). Both, CNS tissue and CSF-ctDNA showed positive mutant droplets for MYD88 p.L265P, while no MYD88 p.V217F mutant droplets were detected. The representative bar chart illustrates the percentage value of mutation allele frequency (MAF) for the CNS tissue and the CSF-ctDNA.